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Differences in human alpha-, beta- and delta-globin gene expression in monkey kidney cells.

Authors
  • Humphries, R K
  • Ley, T
  • Turner, P
  • Moulton, A D
  • Nienhuis, A W
Type
Published Article
Journal
Cell
Publisher
Elsevier
Publication Date
Aug 01, 1982
Volume
30
Issue
1
Pages
173–183
Identifiers
PMID: 6290076
Source
Medline
License
Unknown

Abstract

We have compared the function of the human alpha-, beta- and delta-globin genes using various plasmid expression vectors derived from pBR322. Amplification of recombinants occurred after their introduction, by calcium-phosphate-mediated DNA transfer, into monkey kidney cells that constitutively produce T antigen (COS cells). The human alpha-globin gene promoter functioned independently, but the beta-globin gene promoter was nearly totally dependent on the enhancing activity of the 72 bp direct repeats from the SV40 genome. Furthermore, when the human alpha- and beta-globin genes were linked in the same vector, the alpha promoter was active but the beta promoter was not. Function of the delta-globin gene promoter also depended on the enhancer element. In vectors containing the 72 bp repeats and the beta- or delta-globin gene, the activity of the beta-globin gene was approximately 50 times greater than that of the delta-globin gene, approximating the ratio of beta and delta mRNA observed in normal human bone marrow cells.

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