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Dietary Protein Intake Level Modulates Mucosal Healing and Mucosa-Adherent Microbiota in Mouse Model of Colitis

Authors
  • Vidal-Lletjós, Sandra1
  • Andriamihaja, Mireille1
  • Blais, Anne1
  • Grauso, Marta1
  • Lepage, Patricia2
  • Davila, Anne-Marie1
  • Viel, Roselyne
  • Gaudichon, Claire1
  • Leclerc, Marion2
  • Blachier, François1
  • Lan, Annaïg1
  • 1 (F.B.)
  • 2 (M.L.)
Type
Published Article
Journal
Nutrients
Publisher
MDPI AG
Publication Date
Feb 28, 2019
Volume
11
Issue
3
Identifiers
DOI: 10.3390/nu11030514
PMID: 30823387
PMCID: PMC6471602
Source
PubMed Central
Keywords
License
Green

Abstract

Mucosal healing after an inflammatory flare is associated with lasting clinical remission. The aim of the present work was to evaluate the impact of the amount of dietary protein on epithelial repair after an acute inflammatory episode. C57BL/6 DSS-treated mice received isocaloric diets with different levels of dietary protein: 14% (P14), 30% (P30) and 53% (P53) for 3 ( day 10 ), 6 ( day 13 ) and 21 ( day 28 ) days after the time of colitis maximal intensity. While the P53 diet worsened the DSS- induced inflammation both in intensity and duration, the P30 diet, when compared to the P14 diet, showed a beneficial effect during the epithelial repair process by accelerating inflammation resolution, reducing colonic permeability and increasing epithelial repair together with epithelial hyperproliferation. Dietary protein intake also impacted mucosa-adherent microbiota composition after inflammation since P30 fed mice showed increased colonization of butyrate-producing genera throughout the resolution phase. This study revealed that in our colitis model, the amount of protein in the diet modulated mucosal healing, with beneficial effects of a moderately high-protein diet, while very high-protein diet displayed deleterious effects on this process.

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