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Dibenzazepin hydrochloride as a new spectrophotometric reagent for determination of hydrogen peroxide in plant extracts.

Authors
  • Nagaraja, P
  • Prakash, J S
  • Asha, S C
  • Bhaskara, B L
  • Kumar, S Anil
Type
Published Article
Journal
Environmental Monitoring and Assessment
Publisher
Springer Nature
Publication Date
Oct 01, 2012
Volume
184
Issue
10
Pages
5983–5988
Identifiers
DOI: 10.1007/s10661-011-2395-x
PMID: 22270583
Source
Medline
License
Unknown

Abstract

A rapid, simple, accurate, and sensitive visible spectrophotometric method for the determination of trace amounts of hydrogen peroxide in acidic buffer medium is reported. The proposed method is based on the oxidative coupling of Ampyrone with dibenzazepin hydrochloride by hydrogen peroxide in the buffer medium of pH 4.0 which is catalyzed by ferrous iron. The blue-colored product formed with maximum absorption at 620 nm was found to be stable for 2 h. Beer's law is obeyed for hydrogen peroxide concentration in the range of 0.03-0.42 μg ml(-1). The optimum reaction conditions and other important optical parameters are reported. The molar absorptive and Sandell's sensitivity are found to be 5.89 × 10(4) mol(-1) cm(-1) and 0.57 g/cm(2), respectively. The interference due to diverse ions and complexing agents was studied. The method is successfully applied to the determination of hydrogen peroxide in green plants satisfactorily.

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