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Diagnosis of pulmonary infection with Toxoplasma gondii in immunocompromised HIV-positive patients by real-time PCR.

Authors
  • Petersen, E
  • Edvinsson, B
  • Lundgren, B
  • Benfield, T
  • Evengård, B
Type
Published Article
Journal
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
Publication Date
Jun 01, 2006
Volume
25
Issue
6
Pages
401–404
Identifiers
PMID: 16767490
Source
Medline
License
Unknown

Abstract

The aim of the study presented here was to evaluate the use of PCR for improving the diagnosis of Toxoplasma gondii infection in immunocompromised hosts. Three hundred thirty-two bronchoalveolar lavage (BAL) fluid samples were analyzed by real-time PCR targeting a 529 bp element of T. gondii. In positive samples, the genotype of the parasite was determined by sequence analysis of the GRA6 gene. Positive results were achieved for 2% (7/332) of the samples tested. Genotyping was possible in two samples and revealed GRA6 type II T. gondii. PCR for detecting T. gondii in BAL samples should be performed in all immunosuppressed HIV-positive patients with symptoms of a systemic infection of unknown etiology. Trimethoprim-sulfamethoxazole prophylaxis does not exclude concomitant infection with T. gondii.

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