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Heterologous glucoamylase production with immobilisedKluyveromyces lactiscells in a fluidised bed reactor operating as a two-(liquid–solid) or a three-(gas–liquid–solid) phases system

Authors
Journal
Process Biochemistry
1359-5113
Publisher
Elsevier
Publication Date
Volume
41
Issue
11
Identifiers
DOI: 10.1016/j.procbio.2006.05.020
Keywords
  • Fluidised Bed Reactor
  • Heterologous Protein Production
  • Immobilised Cells
  • Kluyveromyces Lactis

Abstract

Abstract Viable cells of Kluyveromyces lactis, transformed with the glucoamylase gene from Arxula adeninivorans, were entrapped in beads of Ca-alginate and employed on a lab scale in a fluidised bed reactor, operating as a two-(liquid–solid) or three-(gas–liquid–solid) phases system, to produce glucoamylase. The reactor was fed with a non-selective medium containing lactose as carbon source and filled with beads that were checked for size and measured 0.21 ± 0.02 cm in diameter. The performance of the three-phase system, in terms of specific glucoamylase productivity, was twice as high (0.017 U mg cell −1 h −1) than that of the two-phase operating system. Further, higher product concentrations in the outlet were achieved, due to the possibility to enhance the average residence time of the substrate.

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