As an aid to evaluate an existing Staphylococcus aureus control programme implemented on an organic dairy farm, staphylococci isolates from bovine teat skin, milking gloves, milking equipment and bovine milk were identified to species level using the Staph ID 32 API system. Phenotypically and biochemically identified S.aureus isolates were analysed by pulsed-field gel electrophoresis (PFGE) and DNA sequence analysis of the X region of the protein A gene (Spa). PFGE identified two main S. aureus types with two subtypes each. Spa typing also identified two main types; one of which consisted of two subtypes. The results were used to identify possible transmission pathways of S. aureus strains and to address potential points of weakness in the S. aureus mastitis control programme.