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Development of an original procedure for toxic compounds multi-detectionusing an acetylcholinesterase-based biosensors

  • Stepurska, Kateryna
Publication Date
Jun 03, 2016
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Investigations reported in this manuscript are focused on the development of an original approach for the detection of several toxic compounds, mainly aflatoxins and organophosphorus pesticides, using acetylcholinesterase (AChE)-based inhibitory electrochemical biosensors. In a first step, a new potentiometric biosensor using pH Sensitive Field-Effect Transistors (pH-FETs) as transducers was investigated for aflatoxin B1 (AFB1) determination and different elaboration and working parameters were optimized. The proposed biosensor was characterized by high operational stability and reproducibility of the signal during the work as well as during the storage. The biosensor was further evaluated for real samples analysis (wheat, sesame, walnuts and peas) and a mathematical simulation of the potentiometric biosensor response to aflatoxin B1 was proposed for the first time and validated. In a second step, a conductometric biosensor using interdigitated gold microelectrodes was developed. The sensitivity of the biosensor to aflatoxins and other classes of toxic substances, such as organophosphorus pesticides, heavy metals ions, glycoalkaloids, and surfactants, was determined. A new and original procedure, enabling the selective determination of multiclass toxins by applying successive reactivation solutions targeting either irreversible or reversible inhibitors, was finally proposed. Using this method, the electrochemical enzyme inhibitory biosensors could be applied to the analysis of aflatoxins and organophosphorus pesticides, as well as for the determination of total toxicity of the samples

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