Affordable Access

deepdyve-link
Publisher Website

A developmentally regulated Myb domain protein regulates expression of a subset of stage-specific genes in Entamoeba histolytica.

Authors
  • Ehrenkaufer, Gretchen M
  • Hackney, Jason A
  • Singh, Upinder
Type
Published Article
Journal
Cellular Microbiology
Publisher
Wiley (Blackwell Publishing)
Publication Date
Jun 01, 2009
Volume
11
Issue
6
Pages
898–910
Identifiers
DOI: 10.1111/j.1462-5822.2009.01300.x
PMID: 19239479
Source
Medline
License
Unknown

Abstract

Conversion between a cyst and trophozoite stage is essential to disease transmission and pathogenesis in the parasitic protist Entamoeba histolytica. A transcriptomic analysis of E. histolytica cysts and trophozoites has recently been accomplished, but the molecular basis of the regulation of encystation is not known. We have now identified a developmentally regulated Myb protein (belonging to the SHAQKY family of Myb proteins), which controls expression of a subset of amoebic stage-specific genes. Overexpression of the nuclear localized Myb protein resulted in a transcriptome that overlapped significantly with the expression profile of amoebic cysts. Analysis of promoters from genes regulated by the Myb protein identified a CCCCCC promoter motif to which amoebic nuclear protein(s) bind in a sequence-specific manner. Chromatin immunoprecipitation demonstrated that the E. histolytica Myb protein binds to promoters of genes which contain the CCCCCC motif and which are regulated by the Myb protein. This work is the first identification of a transcription factor, which regulates expression of a subset of stage-specific genes in E. histolytica. Identification of transcriptional regulatory networks that control developmental pathways will provide novel insights into the biology of this important human pathogen.

Report this publication

Statistics

Seen <100 times