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The developmental transcriptome of Drosophila melanogaster.

Authors
  • Br, Graveley
  • Angela Brooks
  • Jw, Carlson
  • Mo, Duff
  • Jm, Landolin
  • L, Yang
  • Cg, Artieri
  • Mj, Van Baren
  • N, Boley
  • Bw, Booth
  • Jb, Brown
  • L, Cherbas
  • Ca, Davis
  • A, Dobin
  • R, Li
  • W, Lin
  • Jh, Malone
  • Nr, Mattiuzzo
  • D, Miller
  • D, Sturgill
  • And 27 more
Type
Published Article
Journal
Nature
Publisher
Springer Nature
Volume
471
Issue
7339
Pages
473–479
Identifiers
DOI: 10.1038/nature09715
Source
UCSC Cancer biomedical-ucsc
License
Unknown

Abstract

Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.

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