Affordable Access

Publisher Website

Developmental Toxicity Assessment Using Zebrafish-Based High-Throughput Screening.

Authors
  • Dasgupta, Subham1, 2
  • Simonich, Michael T1
  • Tanguay, Robyn L3
  • 1 Sinnhuber Aquatic Research Laboratory, Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, USA.
  • 2 Department of Biological Sciences, Clemson University, Clemson, SC, USA.
  • 3 Sinnhuber Aquatic Research Laboratory, Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, USA. [email protected].
Type
Published Article
Journal
Methods in molecular biology
Publisher
Clifton, N.J. : Humana Press
Publication Date
Jan 01, 2024
Volume
2707
Pages
71–82
Identifiers
DOI: 10.1007/978-1-0716-3401-1_4
PMID: 37668905
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Zebrafish-based high-throughput screening has been extensively used to study toxicological profiles of individual chemicals and mixtures, identify novel toxicants, and study modes of action to prioritize chemicals for further testing and policy decisions. Within this chapter, we describe a protocol for automated zebrafish developmental high-throughput screening in our laboratory, with emphasis on exposure setups, morphological and behavioral readouts, and quality control. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Report this publication

Statistics

Seen <100 times