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Developmental expression of choline acetyltransferase mRNA in Drosophila.

Authors
  • Carbini, L A
  • Muñoz Maines, V J
  • Salvaterra, P M
Type
Published Article
Journal
Neurochemical research
Publication Date
Nov 01, 1990
Volume
15
Issue
11
Pages
1089–1096
Identifiers
PMID: 2128533
Source
Medline
License
Unknown

Abstract

We have measured the steady state levels of choline acetyltransferase (ChAT, EC 2.3.1.6) mRNA during different developmental stages of Drosophila melanogaster using a ChAT specific cRNA probe. ChAT mRNA was first detected approximately 6-7 h after oviposition, increased until the 1st-2nd larval instar, decreased into early pupal stages and increased again during late pupation, reaching a maximum in adults. Northern analysis showed a major RNA band with a Mr of 4.7 kilobases and Western analysis also showed a single major 75 kD protein band at all developmental stages. Our results support the hypothesis that a major point of regulation of ChAT expression may be at the transcriptional level.

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