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The developmental dynamics of the maize leaf transcriptome.

Authors
  • 1
  • 1 Boyce Thompson Institute, Cornell University, Ithaca, New York, USA.
Type
Published Article
Journal
Nature Genetics
1546-1718
Publisher
Nature Publishing Group
Publication Date
Volume
42
Issue
12
Pages
1060–1067
Identifiers
DOI: 10.1038/ng.703
PMID: 21037569
Source
Medline
License
Unknown

Abstract

We have analyzed the maize leaf transcriptome using Illumina sequencing. We mapped more than 120 million reads to define gene structure and alternative splicing events and to quantify transcript abundance along a leaf developmental gradient and in mature bundle sheath and mesophyll cells. We detected differential mRNA processing events for most maize genes. We found that 64% and 21% of genes were differentially expressed along the developmental gradient and between bundle sheath and mesophyll cells, respectively. We implemented Gbrowse, an electronic fluorescent pictograph browser, and created a two-cell biochemical pathway viewer to visualize datasets. Cluster analysis of the data revealed a dynamic transcriptome, with transcripts for primary cell wall and basic cellular metabolism at the leaf base transitioning to transcripts for secondary cell wall biosynthesis and C(4) photosynthetic development toward the tip. This dataset will serve as the foundation for a systems biology approach to the understanding of photosynthetic development.

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