Affordable Access

Developmental competence of bovine oocytes frozen at different cooling rates.

Authors
  • Otoi, T
  • Tachikawa, S
  • Kondo, S
  • Takagi, M
  • Suzuki, T
Type
Published Article
Journal
Cryobiology
Publication Date
Aug 01, 1994
Volume
31
Issue
4
Pages
344–348
Identifiers
PMID: 7924393
Source
Medline
License
Unknown

Abstract

Experiments were conducted to determine the optimal cooling conditions for improving the developmental competence of in vitro-matured bovine oocytes when frozen in 1.6 M 1,2-propanediol. Bovine oocytes were cooled from 0 degree to -5.5 degrees C at 1 degree C/min, seeded, and then cooled to -30 degrees C at different cooling rates (0.3 degree, 0.6 degree or 0.9 degree C/min) and then plunged into LN2. When frozen-thawed oocytes were fertilized in vitro, the proportions of fertilization and developmental competence did not differ among the three groups frozen at different cooling rates. The findings indicated that a cooling rate of less than 1.0 degree C/min in the freezing procedure did not affect the developmental competence of postthaw in vitro-matured bovine oocytes.

Report this publication

Statistics

Seen <100 times