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Development and validation of a quantitative PCR for rapid and specific detection of California sea lion adenovirus 1 and prevalence in wild and managed populations.

Authors
  • Cortés-Hinojosa, Galaxia1, 2, 3, 4, 5, 6
  • Gulland, Frances M D1, 2, 3, 4, 5, 6
  • Goldstein, Tracey1, 2, 3, 4, 5, 6
  • Venn-Watson, Stephanie1, 2, 3, 4, 5, 6
  • Rivera, Rebecca1, 2, 3, 4, 5, 6
  • Archer, Linda L1, 2, 3, 4, 5, 6
  • Waltzek, Thomas B1, 2, 3, 4, 5, 6
  • Gray, Gregory C1, 2, 3, 4, 5, 6
  • Wellehan, James F X Jr1, 2, 3, 4, 5, 6
  • 1 Departments of Small Animal Clinical Sciences (Cortes-Hinojosa, Archer, Wellehan) and Infectious Diseases and Pathology (Waltzek), College of Veterinary Medicine, University of Florida, Gainesville, FL.
  • 2 Global Health Institute, Duke University, Durham, NC (Gray).
  • 3 The Marine Mammal Center, Sausalito, CA (Gulland).
  • 4 Wildlife Health Center, School of Veterinary Medicine, University of California-Davis, Davis, CA (Goldstein).
  • 5 National Marine Mammal Foundation, San Diego, CA (Venn-Watson).
  • 6 Hubbs-SeaWorld Research Institute, San Diego, CA (Rivera).
Type
Published Article
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Publication Date
Jan 01, 2017
Identifiers
DOI: 10.1177/1040638716689113
PMID: 28166696
Source
Medline
Keywords
License
Unknown

Abstract

California sea lion adenovirus 1 (CSLAdV-1) has been associated with hepatitis and enteritis in several wild and captive populations of diverse pinniped species. Currently available tests have been limited to pan-adenoviral polymerase chain reaction (PCR) followed by sequencing. We present the development of a quantitative probe-hybridization PCR (qPCR) assay for rapid, sensitive, and specific detection of this virus in California sea lions ( Zalophus californianus) and other pinnipeds. This assay did not amplify other mammalian adenoviruses and is able to detect consistently down to 10 viral copies per well. Compared with the gold standard conventional pan-adenovirus PCR/sequencing assay, diagnostic sensitivity and specificity of 100% and 88.2% were found, respectively. The lower diagnostic specificity of this qPCR assay may be the result of the lower limit of detection of this assay compared with the gold standard rather than the result of detection of true false-positives.

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