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Development and validation of headspace Solid-Phase microextraction coupled with gas chromatography (HS-SPME-GC) method for the analysis of zingiber zerumbet L.

Authors
  • Bhavya, M L1
  • Ravi, R2
  • Madhava Naidu, M1
  • 1 Spices, and Flavour science Department.
  • 2 Sensory Science Department, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, 570 020, India. , (India)
Type
Published Article
Journal
Natural product research
Publication Date
Apr 01, 2021
Volume
35
Issue
7
Pages
1221–1225
Identifiers
DOI: 10.1080/14786419.2019.1643863
PMID: 31328550
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography (GC-FID) was explored to determine the fingerprinting characteristic of Zingiber zerumbet L. volatiles to differentiate between different ginger species. The effect of different fibers [polydimethylsiloxane (PDMS, 7 μm), polyacrylate (PA, 85 μm)], different temperature and time on the HS-SPME-GC was investigated by using response surface methodology coupled with full factorial experimental design. The area percentage of the major sesquiterpenes (Zerumbone and α-humulene) were 56.3 ± 4.7% and 47.5 ± 27.2% with PA fiber, respectively at optimum condition of 70 °C and 30 min. Validation of the developed HS-SPME-GC method with limits of detection and quantification for zerumbone was 0.09 and 0.28 μg/g, respectively, demonstrating the suitable sensitivity of HS-SPME-GC method for the quantification of sesquiterpenes. Therefore, the simplicity of HS-SPME-GC makes it a convenient tool for qualitative and quantitative comparison of different ginger species targeting at the marker sesquiterpene molecule, zerumbone.

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