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Development of a simple, sensitive and specific bioassay for interleukin-1 based on the proliferation of RPMI 1788 cells: comparison with other bioassays for IL-1

  • Vandenabeele, Peter
  • Declercq, Wim
  • Libert, Claude
  • Fiers, Walter
Publication Date
Jan 01, 1990
Ghent University Institutional Archive
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The IL-1-dependent proliferation of RPMI 1788, a human EBV-transformed cell line, was used to develop a biological assay system for IL-1. Preparations of rhIL-1-alpha and rhIL-1-beta, as well as rmIL-1-beta exhibited a specific biological activity (50% of the maximal response) between 5.8 x 10(8) and 8.6 x 10(8) U/mg. Remarkably, a 3-5 fold reduced specific biological activity was noticed for rm-IL-1-alpha, viz. 1.7 x 10(8) U/mg. The IL-1-dependent proliferation of RPMI 1788 cells was compared with other IL-1 test systems, such as the IL-1-mediated induction of IL-2 in EL4-NOB-1, LBRM-33-1A5 and thymocytes, and the IL-1-driven induction of cytotoxic activity by PC60 cells, the so-called CIA assay. The cytokine-dependent growth of RPMI 1788 cells is highly specific for IL-1, and no other cytokine tested induced a proliferative response. The presence of high concentrations of rmTNF, rhTNF or rhIL-6 did not interfere with the quantification of IL-1. Additionally, we evaluated the detection of IL-1 in the presence of mitogens, phorbol ester or calcium ionophore, as well as the determination of IL-1 in serum and PF samples of human and murine origin.

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