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Development of a simple multiresidue extraction method for the quantification of a wide polarity range list of pesticides and transformation products in eggs by liquid chromatography and tandem mass spectrometry.

Authors
  • Dufour, Vincent1
  • Wiest, Laure2
  • Slaby, Sylvain3
  • Le Cor, François4
  • Auger, Lucile5
  • Cardoso, Olivier6
  • Curtet, Laurence7
  • Pasquini, Laure8
  • Dauchy, Xavier9
  • Vulliet, Emmanuelle10
  • Banas, Damien11
  • 1 Université de Lorraine, INRAE, URAFPA, F-54000 Nancy, France; Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 Rue de la Doua, F-69100 Villeurbanne, France. Electronic address: [email protected] , (France)
  • 2 Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 Rue de la Doua, F-69100 Villeurbanne, France. Electronic address: [email protected] , (France)
  • 3 Université de Lorraine, INRAE, URAFPA, F-54000 Nancy, France. Electronic address: [email protected] , (France)
  • 4 Université de Lorraine, INRAE, URAFPA, F-54000 Nancy, France; LHN, Laboratoire d'Hydrologie de Nancy, ANSES, 40 Rue Lionnois, 54000 Nancy, France. Electronic address: [email protected] , (France)
  • 5 Université de Lorraine, INRAE, URAFPA, F-54000 Nancy, France; Office Français de la Biodiversité - Montfort, 01330 Birieux, France. Electronic address: [email protected] , (France)
  • 6 Office Français de la Biodiversité - Unité Sanitaire de la Faune, 9 avenue Buffon, 45071 Orléans, France. Electronic address: [email protected] , (France)
  • 7 Office Français de la Biodiversité - Montfort, 01330 Birieux, France. Electronic address: [email protected] , (France)
  • 8 LHN, Laboratoire d'Hydrologie de Nancy, ANSES, 40 Rue Lionnois, 54000 Nancy, France. Electronic address: [email protected] , (France)
  • 9 LHN, Laboratoire d'Hydrologie de Nancy, ANSES, 40 Rue Lionnois, 54000 Nancy, France. Electronic address: [email protected] , (France)
  • 10 Univ Lyon, CNRS, Université Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 Rue de la Doua, F-69100 Villeurbanne, France. Electronic address: [email protected] , (France)
  • 11 Université de Lorraine, INRAE, URAFPA, F-54000 Nancy, France. Electronic address: [email protected] , (France)
Type
Published Article
Journal
Journal of chromatography. A
Publication Date
Sep 27, 2020
Volume
1628
Pages
461447–461447
Identifiers
DOI: 10.1016/j.chroma.2020.461447
PMID: 32822986
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Waterfowl populations have been decreasing in Europe for the last years and pollution appears to be one of the main factors. This study was conducted to develop a single sensitive and robust analytical method for the monitoring of 2 fungicides, 15 herbicides, 3 insecticides and 24 transformation products in wild bird eggs. One of the major challenges addressed was the characterization of chemicals with large logP range (from -1.9 to 4.8). A total of 11 different extraction parameters were tested in triplicate to optimize the extraction protocol, on generic parameters, buffer addition and use of clean-up steps. Quantification was based on matrix-match approach with hen eggs as reference matrix (34 analytes with r²>0.99). Particular attention was payed to matrix effects (-28% on average), quantification limits (0.5 to 25 ng.g-1 dry mass / 0.2 to 7.5 ng.g-1 fresh mass) and extraction yields (46 to 87% with 25 analytes up to 70%) to ensure the relevance of the method and its compatibility with ultra-trace analysis. It led to a simple solid/liquid low temperature partitioning extraction method followed by LC-MS/MS. Analysis of 29 field samples from 3 waterfowl species revealed that eggs were slightly contaminated with pesticides as only one egg presented a contamination (terbutryn, herbicide, 0.7 ng.g-1) and confirmed the relevance of the method. Copyright © 2020. Published by Elsevier B.V.

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