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Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus.

Authors
  • Lyoo, Kwang-Soo1
  • Yeom, Minjoo2
  • Kim, Jungho3
  • Kim, Donghyuk3
  • Ha, Gunwoo3
  • Na, Woonsung2
  • Le, Van Phan4
  • Song, Daesub2
  • 1 Korea Zoonosis Research Institute, Chonbuk National University, Jeonju, Jeollabuk-do, Republic of Korea. , (North Korea)
  • 2 Department of Pharmacy, College of Pharmacy, Korea University, Sejong, Republic of Korea. , (North Korea)
  • 3 BioNote, Hwaseong, Republic of Korea. , (North Korea)
  • 4 Department of Microbiology and Infectious Disease, College of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Vietnam.
Type
Published Article
Journal
Veterinary Record
Publisher
BMJ
Publication Date
Dec 01, 2017
Volume
181
Issue
22
Pages
596–596
Identifiers
DOI: 10.1136/vr.103959
PMID: 28993477
Source
Medline
Keywords
License
Unknown

Abstract

Porcine epidemic diarrhoea virus (PEDV) causes acute and severe watery diarrhoea and dehydration, as well as 50-100 per cent mortality in piglets. For the PEDV diagnosis, a rapid test kit that is specific and sensitive to PEDV is critical to monitor this disease at pig farms. The present study aimed to develop an immunochromatographic assay (ICA) strip test for detecting PEDV in faecal swabs. The newly developed diagnostic test showed a detection limit of 104.0 TCID50/ml of PEDV. Using faecal swab samples, the relative sensitivity and specificity of the ICA kit were 95.0 per cent and 98.6 per cent, respectively, compared with those of real-time RT-PCR. In samples from piglets experimentally infected with PEDV, the results showed 100 per cent agreement with those found by real-time RT-PCR. Our developed test strip will be useful for rapid diagnosis and can be used for epidemiological surveillance of PEDV infection.

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