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Development of a radioimmunoassay for an amino-terminal peptide of pro-opiocortin containing the gamma-MSH region: measurement and characterization in human plasma.

Authors
  • Hope, J
  • Ratter, S J
  • Estivariz, F E
  • McLoughlin, L
  • Lowry, P J
Type
Published Article
Journal
Clinical endocrinology
Publication Date
Sep 01, 1981
Volume
15
Issue
3
Pages
221–227
Identifiers
PMID: 6273026
Source
Medline
Language
English
License
Unknown

Abstract

A radioimmunoassay has been developed for the N-terminal region of human pituitary pro-opiocortin (N-POC), the common precursor protein of ACTH and beta-LPH, using an antiserum which recognizes residues near the gamma-MSH region. The concentrations of greater than 300 ng/l of immunoreactive peptide were determined in unextracted human plasma, the relative molecular mass of the reacting fragments corresponding to a seventy-seven amino acid glycoprotein. The concentrations of immunoreactive N-POC peptides were correlated with those of ACTH in plasma obtained from patients with various disorders of the hypothalamic-pituitary-adrenal axis.

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