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Development of a Rapid Assay for Determining the Relative Abundance of Bacteria

Authors
  • Arlene K. Rowan
  • Russell J. Davenport
  • Jason R. Snape
  • David Fearnside
  • Michael R. Barer
  • Thomas P. Curtis
  • Ian M. Head
Publisher
American Society for Microbiology
Publication Date
Dec 01, 2005
Source
PMC
Keywords
Disciplines
  • Chemistry
  • Earth Science
  • Engineering
License
Unknown

Abstract

A sandwich hybridization assay for high-throughput, rapid, simple, and inexpensive quantification of specific microbial populations was evaluated. The assay is based on the hybridization of a target rRNA with differentially labeled capture and detector probes. Betaproteobacterial ammonia-oxidizing bacteria (AOB) were selected as the target group for the study, since they represent a phylogenetically coherent group of organisms that perform a well-defined geochemical function in natural and engineered environments. Reagent concentrations, probe combinations, and washing, blocking, and hybridization conditions were optimized to improve signal and reduce background. The detection limits for the optimized RNA assay were equivalent to approximately 103 to 104 and 104 to 105 bacterial cells, respectively, for E. coli rRNA and RNA extracted from activated sludge, by using probes targeting the majority of bacteria. Furthermore, the RNA assay had good specificity, permitted discrimination of rRNA sequences that differed by a 2-bp mismatch in the probe target region, and could distinguish the sizes of AOB populations in nitrifying and nonnitrifying wastewater treatment plants.

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