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Development of Monoclonal Antibodies for Diagnosis of Plasmodium vivax.

Authors
  • Linh, Nguyen Thi Phuong1
  • Park, Hyun1
  • Lee, Jinyoung1
  • Liu, Dong-Xu1
  • Seo, Ga-Eun2
  • Sohn, Hae-Jin2
  • Han, Jin-Hee3
  • Han, Eun-Taek3
  • Shin, Ho-Joon2
  • Yeo, Seon-Ju1
  • 1 Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan 54538, Korea. , (North Korea)
  • 2 Department of Microbiology, Ajou University School of Medicine, and Department of Biomedical Science, Graduate School of Ajou University, Suwon 16499, Korea. , (North Korea)
  • 3 Department of Medical Environmental Biology and Tropical Medicine, School of Medicine, Kangwon National University, Chuncheon 24341, Korea. , (North Korea)
Type
Published Article
Journal
The Korean journal of parasitology
Publication Date
Dec 01, 2017
Volume
55
Issue
6
Pages
623–630
Identifiers
DOI: 10.3347/kjp.2017.55.6.623
PMID: 29320817
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Plasmodium lactate dehydrogenase (pLDH) is a strong target antigen for the determination of infection with Plasmodium species specifically. However, a more effective antibody is needed because of the low sensitivity of the current antibody in many immunological diagnostic assays. In this study, recombinant Plasmodium vivax LDH (PvLDH) was experimentally constructed and expressed as a native antigen to develop an effective P. vivax-specific monoclonal antibody (mAb). Two mAbs (2CF5 and 1G10) were tested using ELISA and immunofluorescence assays (IFA), as both demonstrated reactivity against pLDH antigen. Of the 2 antibodies, 2CF5 was not able to detect P. falciparum, suggesting that it might possess P. vivax-specificity. The detection limit for a pair of 2 mAbs-linked sandwich ELISA was 31.3 ng/ml of the recombinant antigen. The P. vivax-specific performance of mAbs-linked ELISA was confirmed by in vitro-cultured P. falciparum and P. vivax-infected patient blood samples. In conclusion, the 2 new antibodies possessed the potential to detect P. vivax and will be useful in immunoassay.

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