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[Development of methodology for living bone imaging.]

Authors
  • Mizuno, Hiroki1
  • Ishii, Masaru1
  • 1 Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, Japan. , (Japan)
Type
Published Article
Journal
Clinical calcium
Publication Date
Jan 01, 2018
Volume
28
Issue
2
Pages
181–185
Identifiers
DOI: CliCa1802181185
PMID: 29371482
Source
Medline
License
Unknown

Abstract

Bone tissue consist of a wide variety of cells such as osteoclasts, osteoblasts and osteocytes which are involved in bone metabolism, hematopoietic cells which can differentiate and mature in the bone marrow, other mesenchymal cells and nerve cells. Recent advances in "fluorescent imaging technology" have made it possible to observe bone tissue alive. And intravital imaging enable us not only to examine the "morphology" but also to analyze the "dynamics" of the cells. We have improved "two-photon microscope" which can observe deep tissue with minimally invasive manner and have established an imaging method to observe the movement of cells in living bone tissue in real time. In this review, we summarize the methodology of intravital imaging, such as the principle of two-photon excitation microscope, method of in vivo imaging of bone, and analysis of acquired imaging data.

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