Affordable Access

Access to the full text

Development of a method for phycocyanin recovery from filamentous cyanobacteria and evaluation of its stability and antioxidant capacity

Authors
  • Aoki, Jinichi1, 2
  • Sasaki, Daisaku3
  • Asayama, Munehiko1, 2
  • 1 Ibaraki University, 3-21-1 Ami, Ibaraki 300-0393, Japan , Ibaraki 300-0393 (Japan)
  • 2 Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho Fuchu-shi, Tokyo 183-8509, Japan , Tokyo 183-8509 (Japan)
  • 3 BioX Chemical Industries Co. Ltd., 2-20-11 Inokuchidai, Nishi-ku, Hiroshima 733-0844, Japan , Hiroshima 733-0844 (Japan)
Type
Published Article
Journal
BMC Biotechnology
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Jun 16, 2021
Volume
21
Issue
1
Identifiers
DOI: 10.1186/s12896-021-00692-9
Source
Springer Nature
Keywords
Disciplines
  • Research
License
Green

Abstract

BackgroundMost commercial phycocyanins are extracted from a filamentous cyanobacterium, Arthrospira (Spirulina) platensis. Owing to the expenses of culture and complexities of the physical and chemical methods of phycocyanin purification, a more effective and simple method is required.ResultsWe developed a new method for efficiently recovering the blue pigment protein, phycocyanin, from unique filamentous cyanobacteria, Pseudanabaena sp. ABRG5-3 and Limnothrix sp. SK1-2-1. The cells were cultivated in economy medium BG11 and lysed by adding water in a 1:16 ratio of wet cells to water. After extraction and purification, 28–30% dry cell weight of phycocyanin was obtained and its purity was confirmed. The stabilities of the phycocyanins at different pH in the presence of high temperature and light conditions and their antioxidant abilities were assessed. Results indicated that the phycocyanins were stable and possessed antioxidant properties. Interestingly, the Pseudanabaena phycocyanin was less likely to deteriorate under acidic conditions.ConclusionsOverall, we developed a promising and novel method for producing high functional phycocyanin concentrations at a low cost. The possibilities of adapting this new phycocyanin biorefinery to unique bioreactor utilization have also been discussed.

Report this publication

Statistics

Seen <100 times