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Development of metabolic function biomarkers in the common marmoset, Callithrix jacchus.

Authors
  • Ziegler, Toni E
  • Colman, Ricki J
  • Tardif, Suzette D
  • Sosa, Megan E
  • Wegner, Fredrick H
  • Wittwer, Daniel J
  • Shrestha, Hemanta
Type
Published Article
Journal
American Journal of Primatology
Publisher
Wiley (John Wiley & Sons)
Publication Date
May 01, 2013
Volume
75
Issue
5
Pages
500–508
Identifiers
DOI: 10.1002/ajp.22126
PMID: 23447060
Source
Medline
License
Unknown

Abstract

Metabolic assessment of a non-human primate model of metabolic syndrome and obesity requires the necessary biomarkers specific to the species. While the rhesus monkey has a number of specific assays for assessing metabolic syndrome, the marmoset does not. Furthermore, the common marmoset (Callithrix jacchus) has a small blood volume that necessitates using a single blood volume for multiple analyses. The common marmoset holds a great potential as an alternative primate model for the study of human disease but assay methods need to be developed and validated for the biomarkers of metabolic syndrome. Here we report on the adaptation, development, and validation of commercially available immunoassays for common marmoset samples in small volumes. We have performed biological validations for insulin, adiponectin, leptin, and ghrelin to demonstrate the use of these biomarkers in examining metabolic syndrome and other related diseases in the common marmoset.

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