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Development of a luminescence immunoassay for follitropin suitable for clinical routine.

Authors
  • Biro, G
  • Samira, A
  • Butz, H
  • Leicht, E
  • Weinges, K F
Type
Published Article
Journal
European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies
Publication Date
Mar 01, 1991
Volume
29
Issue
3
Pages
189–192
Identifiers
PMID: 1906350
Source
Medline
License
Unknown

Abstract

We developed a luminescence immunoassay (LIA) for follitropin, based on the synthesis of a follitropin-N-(4-aminobutyl)-N-ethylisoluminol conjugate. The luminescence tracer was purified by gel chromatography. Antibody-bound and non-bound tracer fractions were separated by using a second antibody reagent bound to magnetic particles. The assay can be performed within 24 hours and is sufficiently sensitive for the measurement of all clinically relevant follitropin concentrations including the subnormal range.

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