Affordable Access

deepdyve-link
Publisher Website

Development of an immunochromatographic strip for antibody detection of pseudorabies virus in swine.

Authors
  • Li, Xiujie1
  • Sun, Yaning1
  • Yang, Suzhen2
  • Wang, Yinbiao1
  • Yang, Jifei1
  • Liu, Yunchao1
  • Jin, Qianyue1
  • Li, Xuewu1
  • Guo, Chengliu1
  • Zhang, Gaiping2
  • 1 Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China (XJ Li, Sun, S Yang, Wang, J Yang, Liu, Jin, XW Li, Guo, Zhang)College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China (Zhang)Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, People's Republic of China (XJ Li, S Yang, Zhang). , (China)
  • 2 Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China (XJ Li, Sun, S Yang, Wang, J Yang, Liu, Jin, XW Li, Guo, Zhang)College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People's Republic of China (Zhang)Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, People's Republic of China (XJ Li, S Yang, Zhang) [email protected] [email protected] , (China)
Type
Published Article
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Publication Date
Nov 01, 2015
Volume
27
Issue
6
Pages
739–742
Identifiers
DOI: 10.1177/1040638715611442
PMID: 26472745
Source
Medline
Keywords
License
Unknown

Abstract

An immunochromatographic strip was developed for the serological detection of pseudorabies virus (PRV) in swine. In the strip, the expressed protein of gB, one of the glycoproteins of PRV, labeled with colloidal gold, was used as the detector; staphylococcal protein A and swine anti-pseudorabies virus antibody were blotted on nitrocellulose membrane for the test and control lines, respectively. The specificity of the strip was 98.1%, and the sensitivity of the strip with reference anti-PRV serum was 96.0%. Swine serum samples (296) were collected to evaluate the characteristics of the strip in comparison with an existing commercial kit. The agreement was 93.6%. Furthermore, the dipstick assay based on the strip is rapid (5 min) and easy to perform with no requirement of professional skills, reagents, or equipment. This suggests that the immunochromatographic strip is an acceptable alternative for use in clinical laboratories lacking specialized equipment and for field diagnosis.

Report this publication

Statistics

Seen <100 times