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Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magenetic particles and fully automated system

Authors
  • Sato, Reiko1
  • Takeyama, Haruko1
  • Tanaka, Tsuyoshi1
  • Matsunaga, Tadashi1
  • 1 Tokyo University of Agriculture and Technology, Department of Biotechnology, 2-24-16, Naka-cho Koganei, Tokyo, 184-8588, Japan , Tokyo
Type
Published Article
Journal
Applied Biochemistry and Biotechnology
Publisher
Springer-Verlag
Publication Date
Mar 01, 2001
Volume
91-93
Issue
1-9
Pages
109–116
Identifiers
DOI: 10.1385/ABAB:91-93:1-9:109
Source
Springer Nature
Keywords
License
Yellow

Abstract

A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contians a reaction station, tip rack, and an eight-tip pipettor that is alble to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.

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