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Development and Evaluation of a Reverse-Entry Ion Source Orbitrap Mass Spectrometer

Authors
  • Poltash, Michael L.1
  • McCabe, Jacob W.1
  • Patrick, John W.1
  • Laganowsky, Arthur1
  • Russell, David H.1
  • 1 Texas A&M University, Department of Chemistry, College Station, TX, 77843, USA , College Station (United States)
Type
Published Article
Journal
Journal of the American Society for Mass Spectrometry
Publisher
Springer-Verlag
Publication Date
May 23, 2018
Volume
30
Issue
1
Pages
192–198
Identifiers
DOI: 10.1007/s13361-018-1976-0
Source
Springer Nature
Keywords
License
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Abstract

As a step towards development of a high-resolution ion mobility mass spectrometer using the orbitrap mass analyzer platform, we describe herein a novel reverse-entry ion source (REIS) coupled to the higher-energy C-trap dissociation (HCD) cell of an orbitrap mass spectrometer with extended mass range. Development of the REIS is a first step in the development of a drift tube ion mobility-orbitrap MS. The REIS approach retains the functionality of the commercial instrument ion source which permits the uninterrupted use of the instrument during development as well as performance comparisons between the two ion sources. Ubiquitin (8.5 kDa) and lipid binding to the ammonia transport channel (AmtB, 126 kDa) protein complex were used as model soluble and membrane proteins, respectively, to evaluate the performance of the REIS instrument. Mass resolution obtained with the REIS is comparable to that obtained using the commercial ion source. The charge state distributions for ubiquitin and AmtB obtained on the REIS are in agreement with previous studies which suggests that the REIS-orbitrap EMR retains native structure in the gas phase. Graphical Abstractᅟ

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