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Development and Evaluation of the Protective Efficacy of Novel Marek's Disease Virus Rispens Vector Vaccines Against Infectious Bursal Disease.

Authors
  • Ishihara, Yukari1
  • Esaki, Motoyuki1
  • Saitoh, Shuji1
  • Sato, Takanori1
  • Yasuda, Atsushi1
  • 1 Ceva Animal Health (Japan Campus), 1-6 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. , (Japan)
Type
Published Article
Journal
Avian Diseases
Publisher
BioOne (American Association of Avian Pathologists)
Publication Date
Sep 01, 2016
Volume
60
Issue
3
Pages
618–627
Identifiers
DOI: 10.1637/11352-122215-Reg.1
PMID: 27610721
Source
Medline
Keywords
License
Unknown

Abstract

Infectious bursal disease (IBD) is a major disease affecting the poultry industry and is caused by infection with IBD virus (IBDV). To develop a novel vaccine to prevent IBD in chickens, recombinant Marek's disease virus Rispens viruses carrying the VP2 gene of IBDV driven by five different promoters (Rispens/IBD) were constructed using homologous recombination and a bacterial artificial chromosome (BAC). Rispens/IBD driven by the chicken beta-actin (Bac) promoter (Rispens/Bac-IBD), Rous sarcoma virus promoter, or simian virus 40 promoter were administered to 1-day-old SPF chicks, and the protective efficacy against IBDV was evaluated by challenging chicks with virulent IBDV. As a result, Rispens/Bac-IBD showed the best protection (87%). Next, we constructed the virus driven by the Bac-derived Coa5 promoter (Rispens/Coa5-IBD) for a secondary in vivo trial using commercial layer chickens since Rispens/Bac-IBD was thought to be genetically unstable. Rispens/Coa5-IBD showed stability in vitro and exhibited better antibody production and protection during challenge against virulent IBDV at both 5 (95%) and 7 wk of age (91%) compared with that of Rispens/Bac-IBD (90% at 5 wk of age and 84% at 7 wk of age). Thus, Rispens/Coa5-IBD may be a novel promising vaccine against IBD and virulent Marek's disease.

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