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Development of an encapsulation-vitrification protocol for Rubia akane (nakai) hairy roots: a comparison with non-encapsulation.

Authors
  • Shin, D-J1
  • Lee, H-E2
  • Bae, C-H3
  • Park, S-U4
  • Kang, H-N1
  • Kim, H-H5
  • 1 Species Restoration Center, Korea National Park Service, Gurye, Korea. , (North Korea)
  • 2 Department of Well-being Resources, Sunchon National University, Suncheon, Korea. , (North Korea)
  • 3 National Institute of Biological Resources, 42 Nanji-ro, Seo-gu, Incheon, Korea. , (North Korea)
  • 4 Division of Plant Science and Resources, Chungnam National University, Daejeon, Korea. , (North Korea)
  • 5 Department of Well-being Resources, Sunchon National University, Suncheon, Korea. [email protected] , (North Korea)
Type
Published Article
Journal
Cryo letters
Publication Date
Jan 01, 2014
Volume
35
Issue
5
Pages
377–384
Identifiers
PMID: 25397952
Source
Medline
License
Unknown

Abstract

Even though encapsulation provided some degree of protection from the cytotoxicity of vitrification solutions to cytotoxicity-sensitive R. akane hairy roots, an overall higher post-cryopreservation regrowth was obtained using the droplet-vitrification procedure under optimized conditions. This result implies that this sensitive material was not sufficiently cryoprotected, and thus, rapid cooling and warming using foil strips was more efficient than cryopreservation of encapsulated samples.

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