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Development of DNA-mediated transformation systems for plants.

Authors
  • Pasternak, J J1
  • Gruber, M Y
  • Thompson, J E
  • Glick, B R
  • 1 Biology Department, University of Waterloo, Ontario, Canada. , (Canada)
Type
Published Article
Journal
Biotechnology Advances
Publisher
Elsevier
Publication Date
Jan 01, 1983
Volume
1
Issue
1
Pages
1–15
Identifiers
PMID: 14544242
Source
Medline
Language
English
License
Unknown

Abstract

The genetic engineering of plants by DNA-mediated gene transfer requires that efficient transformation systems be developed. Considerable progress has been made in manipulating the Ti plasmid of Agrobacterium tumefaciens as a vehicle for delivery of foreign genes into protoplasts of dicotyle-donous plants. Part of the Ti plasmid, the T-DNA, can be incorporated into the genome of the host cell; the T-DNA can carry a foreign DNA sequence which co-integrates with it; under normal conditions, the tumorigenic-causing portion of the T-DNA can be inactivated so that transformed protoplasts can be regenerated and T-DNA with an inserted foreign gene can be stably maintained during regeneration, meiosis and gamete formation. A foreign gene has yet to be expressed in regenerated plants although a T-DNA gene for opine synthesis can function in regenerates. Developing a more ubiquitous transformation system for monocotyledons is further from fruition. Based on transformation systems for simple eukaryotic organisms, it is reasonable to expect that a DNA vector which is capable of amplifying a novel plant gene and which contains both a drug resistance marker to facilitate the selection of transformed plant protoplasts and a species-specific autonomously replicating sequence to ensure the stable maintenance of the input gene in the recipient cell can be constructed.

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