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Development and characterization of monoclonal antibodies against mouse TSLP.

Authors
  • Li, Xiangqun
  • Han, Xue
  • Wang, Shengdian
  • Duan, Ziyuan
Type
Published Article
Journal
Hybridoma
Publisher
Mary Ann Liebert
Publication Date
Oct 01, 2010
Volume
29
Issue
5
Pages
425–430
Identifiers
DOI: 10.1089/hyb.2010.0011
PMID: 21050044
Source
Medline
License
Unknown

Abstract

Epithelial-derived thymic stromal lymphopoietin (TSLP) is an IL-7-like cytokine that is mainly produced by epithelial cells. It has been shown to play a key role in the development of Th2-type allergic inflammation. Commercial antibodies available against TSLP can only be used in Western blot assay, which further limits investigation of its function. Here we efficiently generated a panel of anti-mouse TSLP monoclonal antibodies in rats with DNA priming-protein boosting strategy. Overall, four MAb strains (4B12, 4E11, 5D7, and 6H10) were obtained and their characterizations were identified. The MAbs can specifically bind to TSLP according to the ELISA and FACS assays. It was found that they recognized distinct epitopes. They are useful in detecting TSLP expression in the tissue by immunoblotting and in the cytoplasm by intracellular staining assay. Thus, these antibodies will be valuable tools for studying TSLP biological functions.

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