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Development and application of a urine drug concentration method for the evaluation of oral absorption of ibandronate in clinical patients.

Authors
  • An, Jing1, 2
  • Jiang, Ye1
  • Lu, Yanfang3
  • Zhang, Lin1
  • Xu, Meng3
  • Hou, Zili1
  • 1 Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang City, Hebei Province 050017, PR China. , (China)
  • 2 Department of Pharmacy, Hebei General Hospital, 348 West Heping Road, Shijiazhuang City, Hebei Province 050051, PR China. , (China)
  • 3 Department of Pharmacy, the Second Hospital of Hebei Medical University, 215 West Heping Road, Shijiazhuang City, Hebei Province 050051, PR China. , (China)
Type
Published Article
Journal
Bioanalysis
Publisher
"Future Science, LTD"
Publication Date
Aug 08, 2019
Identifiers
DOI: 10.4155/bio-2018-0223
PMID: 31392890
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Aim: A urine drug concentration method was developed for the evaluation of oral ibandronte absorption based on the fact that ibandronate is excreted unchanged by the kidneys. Methodology: Ibandronate was isolated from the urine matrix by coprecipitated with 2.5 M CaCl2 and 1 M K2HPO4 in basic conditions. After a liquid-liquid extraction, the analytes were derivatized with trimethylsilydiazomethane prior to detection. Results: The calibration curves exhibited excellent linearity (r > 0.99) between 1 and 250 ng/ml in human urine. Ibandronate was recovered >86.5% with the inter- and intraday relative standard deviations less than 15%. Conclusion: The method is selective, accurate, practical and was successfully applied to study the influence of vitamin D3 on the absorption of ibandronate.

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