Affordable Access

deepdyve-link
Publisher Website

Development of an analytical method to detect short-chain fatty acids by SPME-GC-MS in samples coming from an in vitro gastrointestinal model.

Authors
  • Douny, Caroline1
  • Dufourny, Sandrine2
  • Brose, François3
  • Verachtert, Pauline4
  • Rondia, Pierre5
  • Lebrun, Sarah6
  • Marzorati, Massimo7
  • Everaert, Nadia8
  • Delcenserie, Véronique9
  • Scippo, Marie-Louise10
  • 1 Department of Food Sciences, Laboratory of Food Analysis, FARAH - Veterinary Public Health, University of Liège, B43bis, Bld de Colonster 20, Sart-Tilman, B-4000 Liège, Belgium. Electronic address: [email protected] , (Belgium)
  • 2 Production and sectors Department, Animal Nutrition and Sustainability Unit, Walloon Agricultural Research Center, 8, rue de Liroux, B-5030 Gembloux, Belgium. Electronic address: [email protected] , (Belgium)
  • 3 Department of Food Sciences, Laboratory of Food Analysis, FARAH - Veterinary Public Health, University of Liège, B43bis, Bld de Colonster 20, Sart-Tilman, B-4000 Liège, Belgium. Electronic address: [email protected] , (Belgium)
  • 4 Department of Food Sciences, Laboratory of Food Analysis, FARAH - Veterinary Public Health, University of Liège, B43bis, Bld de Colonster 20, Sart-Tilman, B-4000 Liège, Belgium. , (Belgium)
  • 5 Production and sectors Department, Animal Nutrition and Sustainability Unit, Walloon Agricultural Research Center, 8, rue de Liroux, B-5030 Gembloux, Belgium. Electronic address: [email protected] , (Belgium)
  • 6 Department of Food Sciences, Laboratory of Food Quality Management, FARAH - Veterinary Public Health, University of Liège, B43bis, Bld de Colonster 20, Sart-Tilman, B-4000 Liège, Belgium. Electronic address: [email protected] , (Belgium)
  • 7 Prodigest Bvba, Gent, Belgium; CMET, University of Ghent, Belgium; AgroBioChem Department, Precision Livestock and Nutrition Unit, TERRA, University of Liège, Gembloux, Belgium. Electronic address: [email protected] , (Belgium)
  • 8 CMET, University of Ghent, Belgium; AgroBioChem Department, Precision Livestock and Nutrition Unit, TERRA, University of Liège, Gembloux, Belgium. Electronic address: [email protected] , (Belgium)
  • 9 Department of Food Sciences, Laboratory of Food Quality Management, FARAH - Veterinary Public Health, University of Liège, B43bis, Bld de Colonster 20, Sart-Tilman, B-4000 Liège, Belgium. Electronic address: [email protected] , (Belgium)
  • 10 Department of Food Sciences, Laboratory of Food Analysis, FARAH - Veterinary Public Health, University of Liège, B43bis, Bld de Colonster 20, Sart-Tilman, B-4000 Liège, Belgium. Electronic address: [email protected] , (Belgium)
Type
Published Article
Journal
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Publication Date
Jun 07, 2019
Volume
1124
Pages
188–196
Identifiers
DOI: 10.1016/j.jchromb.2019.06.013
PMID: 31212236
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Short chain fatty acids (SCFA) are end-products of intestinal bacterial fermentation. The concentrations of fermentation metabolites are closely related to the microbial activity that occurs in various digestive compartments. The fermentation products may vary qualitatively and quantitatively, especially within the colon. The Simulator of the Human Intestinal Microbial Ecosystem (SHIME), an in vitro dynamic and multicompartment model of the human intestinal tract, can be adapted to mimic the piglet gastrointestinal tract. In this context, a quantitative method, based on solid phase microextraction gas chromatography coupled to mass spectrometry (SPME-GC-MS), was developed for the determination of seven short chain fatty acids, i.e. acetic, propionic, butyric, isobutyric, valeric, isovaleric and hexanoic acids, in samples coming from this experimental in vitro gastrointestinal model. The advantage of the SPME-GC-MS technique is that the seven compounds could be determined in a single run, after a simple and rapid sample treatment, without any other extraction than the automatic SPME. The developed method was validated in accordance to the European and US FDA guidelines and showed good specificity/selectivity. In addition, limits of detection and quantification ranged from 8 to 72 mg L-1 and from 16 to 144 mg L-1, respectively. Two internal quality control samples spiked at different concentrations were analyzed to assess the trueness of the developed method, which ranged between 97.7 and 122.4% of the expected value, for the seven compounds analyzed. The method was successfully applied to twenty samples coming from a gastrointestinal model, with different inocula. The developed method might be used as a general method for measuring SCFA in biological samples. Copyright © 2019 Elsevier B.V. All rights reserved.

Report this publication

Statistics

Seen <100 times