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Development of an amperometric polyphenol biosensor based on fungal laccase immobilized on nitrocellulose membrane.

Authors
Type
Published Article
Journal
Artificial cells, blood substitutes, and immobilization biotechnology
Publication Date
Volume
40
Issue
1-2
Pages
163–170
Identifiers
DOI: 10.3109/10731199.2011.637926
PMID: 22192070
Source
Medline

Abstract

A method is described for construction of an amperometric polyphenol biosensor employing nitrocellulose membrane-bound laccase purified from cell-free extract of Ganoderma lucidum onto a Pt electrode. The biosensor showed optimum response within 10s, at 0.4 V in 0.1M acetate buffer, pH 6.0, and 35°C. Detection limit of the biosensor was 3.0 × 10(-8)M. Analytical recovery of added guaiacol was 97.00%. Within batch and between batch coefficients of variation were <0.97% and <1.26%, respectively. The sensor measured total phenolic content in fruit juices and alcoholic beverages. The enzyme electrode was used 100 times over 4 months, when stored at 4°C.

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