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Determining spa-type of methicillin-resistant Staphylococcus aureus (MRSA) via high-resolution melting (HRM) analysis, Shiraz, Iran

Authors
  • Hashemizadeh, Zahra1
  • Bazargani, Abdollah1
  • Kalantar-Neyestanaki, Davood2, 2
  • Mohebi, Samane1
  • Hadi, Nahal1, 3
  • 1 Shiraz University of Medical Science, Shiraz, Iran , Shiraz (Iran)
  • 2 Kerman University of Medical Sciences, Kerman, Iran , Kerman (Iran)
  • 3 Shiraz University of Medical Sciences, Shiraz, Iran , Shiraz (Iran)
Type
Published Article
Journal
BMC Research Notes
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Feb 24, 2020
Volume
13
Issue
1
Identifiers
DOI: 10.1186/s13104-020-04948-z
Source
Springer Nature
Keywords
License
Green

Abstract

ObjectivesMolecular typing methods are useful for rapid detection and control of a disease. Recently, the use of high-resolution melting (HRM) for spa typing of MRSA isolates were reported. This technique is rapid, inexpensive and simple for genotyping and mutation screening in DNA sequence. The aim of this study was to evaluate the ability of HRM-PCR to analysis spa genes amongst MRSA isolates.ResultsA total of 50 MRSA isolates were collected from two teaching hospitals in Shiraz, Iran. The isolates were confirmed as MRSA by susceptibility to cefoxitin and detection of mecA gene using PCR. We used HRM analysis and PCR-sequencing method for spa typing of MRSA isolates. In total, 15 different spa types were discriminate by HRM and sequencing method. The melting temperature of the 15 spa types, using HRM genotyping were between 82.16 and 85.66 °C. The rate of GC % content was 39.4–46.3. According to the results, spa typing of 50 clinical isolates via PCR-sequencing and HRM methods were 100% similar. Consequently, HRM method can easily identify and rapidly differentiate alleles of spa genes. This method is faster, less laborious and more suitable for high sample at lower cost and risk of contamination.

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