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Determining the optimal thymidine concentration for growing Thy- Escherichia coli strains.

Authors
  • Molina, F1
  • Jiménez-Sánchez, A
  • Guzmán, E C
  • 1 Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, E-06080 Badajoz, Spain.
Type
Published Article
Journal
Journal of bacteriology
Publication Date
June 1998
Volume
180
Issue
11
Pages
2992–2994
Identifiers
PMID: 9603893
Source
Medline
License
Unknown

Abstract

Changes of thymidine concentration in the growth medium affect the chromosome replication time of Thy- strains without at the same time causing a detectable difference in the growth rate (R. H. Pritchard and A. Zaritsky, Nature 226:126-131, 1970). Consequently, the optimal thymidine concentration cannot be determined by ascertaining which concentration produces the highest growth rate. Here we present a method for determining the optimal thymidine concentration of any Thy- Escherichia coli strain. Using this method, we found that the E. coli "wild-type" strain MG1655 has a partial Thy- phenotype.

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