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Determining the optimal thymidine concentration for growing Thy- Escherichia coli strains.

Authors
  • 1
Type
Published Article
Journal
Journal of Bacteriology
0021-9193
Publisher
American Society for Microbiology
Publication Date
Volume
180
Issue
11
Pages
2992–2994
Identifiers
PMID: 9603893
Source
Medline

Abstract

Changes of thymidine concentration in the growth medium affect the chromosome replication time of Thy- strains without at the same time causing a detectable difference in the growth rate (R. H. Pritchard and A. Zaritsky, Nature 226:126-131, 1970). Consequently, the optimal thymidine concentration cannot be determined by ascertaining which concentration produces the highest growth rate. Here we present a method for determining the optimal thymidine concentration of any Thy- Escherichia coli strain. Using this method, we found that the E. coli "wild-type" strain MG1655 has a partial Thy- phenotype.

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