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Determining the Effect of Preparation and Storage: An Effort to Streamline Platelet Components as a Source of Growth Factors for Clinical Application.

Authors
Type
Published Article
Journal
Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur̈ Transfusionsmedizin und Immunham̈atologie
Publication Date
Volume
42
Issue
3
Pages
174–180
Identifiers
DOI: 10.1159/000371504
PMID: 26195931
Source
Medline
Keywords
  • Buffy Coat
  • Growth Factors
  • Platelet Transfusion
  • Plateletpheresis

Abstract

The buffy coat method is suitable as preparation method for PRP in most settings. The double freeze thaw technique is better suited as concentration technique as it causes lysis of both platelets and white blood cells for releasing growth factors and is easier to perform. Growth factors are not stable in plasma, thus PRP should be frozen immediately after preparation.

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