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Determination of selected antioxidants in Melissae herba by isotachophoresis and capillary zone electrophoresis in the column-coupling configuration.

Authors
  • Safra, Jirí
  • Pospísilová, Marie
  • Honegr, Jan
  • Spilková, Jirina
Type
Published Article
Journal
Journal of Chromatography A
Publisher
Elsevier
Publication Date
Nov 09, 2007
Volume
1171
Issue
1-2
Pages
124–132
Identifiers
PMID: 17920611
Source
Medline
License
Unknown

Abstract

A method utilising isotachophoresis and capillary zone electrophoresis in the column coupling configuration with UV detection at 320 nm was developed for separation and determination of five phenolic acids (rosmarinic, p-coumaric, ferulic, caffeic and chlorogenic) and flavonoid quercitrin in a methanolic extract of Melissae herba. The proposed method has been validated with correlation coefficients from 0.9842 to 0.9988, RSD values between 0.39% and 0.83% for migration times and between 0.40% and 2.05% for peak areas.

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