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Determination of salsolinol, norsalsolinol, and twenty-one biogenic amines using micellar electrokinetic capillary chromatography-electrochemical detection.

Authors
  • Kuklinski, Nicholas J1
  • Berglund, E Carina
  • Engelbreksson, Johan
  • Ewing, Andrew G
  • 1 Department of Chemistry, The Pennsylvania State University, PA, USA.
Type
Published Article
Journal
Electrophoresis
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jun 01, 2010
Volume
31
Issue
11
Pages
1886–1893
Identifiers
DOI: 10.1002/elps.200900761
PMID: 20446293
Source
Medline
License
Unknown

Abstract

Micellar electrokinetic chromatography coupled to amperometric electrochemical detection was used to resolve and then quantify biogenic amines and metabolites within the fruit fly Drosophila melanogaster. A new separation scheme was devised to allow resolution of 24 compounds of interest. This was accomplished by precisely controlling the amount of base added to the background buffer, optimizing the resolution of the separation, and then calculating the pH. Here we focused on measurements of six of the analytes that are thought to be involved in the response to alcohol, dopamine, salsolinol, norsalsolinol, N-acetyloctopamine, octopamine, and N-acetyldopamine. These were identified and quantified within the fly head. We believe that the identification of salsolinol and norsalsolinol in the fly brain is novel.

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