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Determination of riboflavin, flavin mononucletide and flavin adenine dinucleotide in biological tissues by capillary zone electrophoresis and laser-induced fluorescence detection.

Authors
  • Pérez-Ruiz, T
  • Martínez-Lozano, C
  • Sanz, A
  • Bravo, E
Type
Published Article
Journal
Electrophoresis
Publication Date
Apr 01, 2001
Volume
22
Issue
6
Pages
1170–1174
Identifiers
PMID: 11358143
Source
Medline
License
Unknown

Abstract

The separation of riboflavin, flavin mononucleotide and flavin adenine dinucleotide was investigated by capillary zone electrophoresis using laser-induced fluorescence detection. In the systematic approach developed, the differential electrophoretic mobilities were first maximized by adjusting the pH. Increasing the buffer concentration improved the separation at the expense of migration times. A buffer consisting of 50 mM phosphate adjusted to pH 8.5 was found to provide a very efficient and stable electrophoretic system. Responses were linear within the range 0.1-100 micromol L(-1), and the detection limits of B2 vitamers were 0.23 nmol L(-1) or less. The method was successfully applied to a variety of biological tissues from different animals.

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