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Determination of Rheumatoid Factors by ELISA.

Authors
  • Trier, Nicole Hartwig1
  • Houen, Gunnar2, 3
  • 1 Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark. [email protected] , (Denmark)
  • 2 Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark. , (Denmark)
  • 3 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark. , (Denmark)
Type
Published Article
Journal
Methods in molecular biology (Clifton, N.J.)
Publication Date
Jan 01, 2019
Volume
1901
Pages
263–270
Identifiers
DOI: 10.1007/978-1-4939-8949-2_23
PMID: 30539586
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

IgM and IgA autoantibodies binding to IgG are called rheumatoid factors (RFs) and occur with high frequency in rheumatoid arthritis (RA) and with lower frequency in other autoimmune diseases. RFs have diagnostic and prognostic value in RA, but they also have a high potential to cause false positive reactions in other immunoassays, especially sandwich assays. For these reasons it is imperative to be able to measure RFs in serum samples from patients suspected of RA or other autoimmune diseases and in serum samples to be analyzed by sandwich immunoassay for various clinical parameters. Here, a simple ELISA for IgM and IgA RFs is described.

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