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Determination of quinolones in water samples by solid-phase extraction and liquid chromatography with fluorimetric detection.

Authors
  • Prat, M D
  • Benito, J
  • Compañó, R
  • Hernández-Arteseros, J A
  • Granados, M
Type
Published Article
Journal
Journal of Chromatography A
Publisher
Elsevier
Publication Date
Jul 02, 2004
Volume
1041
Issue
1-2
Pages
27–33
Identifiers
PMID: 15281251
Source
Medline
License
Unknown

Abstract

A method is reported for the determination, in water samples, of 10 quinolones which are used as veterinary drugs. Analytes are isolated from samples by solid-phase extraction (SPE) and analysed by reversed-phase high-performance liquid chromatography using fluorimetric detection. A solid-phase extraction procedure based on retention on HBL OASIS cartridges and elution with a mixture of acetonitrile-water in basic medium is suitable for pre-concentration of the analytes. Pre-concentration factors up to 250 can be obtained. The quinolones are separated with an octyl silica-based column and mobile phases consisting of aqueous oxalic acid solutions and acetonitrile mixtures. The attained detection limits of the whole process are in the ng l(-1) level when 250 ml of water sample is processed. Recovery rates, from natural water samples spiked at 2060 ng l(-1) level, range from 70 to 100% and common standard deviation are about 6-12%.

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