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Determination of partial specific volume and absolute concentration by densimetry.

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PMC
Keywords
  • Research Article
Disciplines
  • Biology

Abstract

The apparent partial specific volume, phi, of a protein (or other macromolecule) and the absolute concentration, c, can be determined from the densimetry of solutions and diffusates in 1H2O , 2H2O and 1H2O/2H2O mixtures if the ratios of concentrations (relative concentrations) are known. The densimetry method allows a non-destructive check on total macromolecule concentrations without prior knowledge of specific u.v.-absorption coefficient. The method may be especially useful in small-angle neutron-scattering studies.

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