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Determination of nicotinamide-adenine dinucleotide and thiazole-4-carboxamide-adenine dinucleotide in human leukocytes by reversed-phase high-performance liquid chromatography.

Authors
  • Saunders, P P
  • Alvarez, E
  • Kantarjian, H M
Type
Published Article
Journal
Journal of chromatography
Publication Date
May 20, 1992
Volume
577
Issue
1
Pages
37–41
Identifiers
PMID: 1400744
Source
Medline
License
Unknown

Abstract

A high-performance liquid chromatographic assay for cellular nicotinamide-adenine dinucleotide and thiazole-4-carboxamide-adenine dinucleotide is presented that is appropriate for analysis of these dinucleotides in extracts of Ficoll-purified human leukemic cells. The separation, which is effected by reversed-phase chromatography, is highly reproducible and the limit of quantitation is as low as 10-15 pmol. The stability of these compounds in neutralized perchloric acid extracts is addressed and the applicability of the procedure to clinical specimens is demonstrated.

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