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Determination of meloxicam using europium sensitized luminescence in the presence of co-luminescence reagents.

Authors
  • Al-Kindy, Salma Muhamed Z1
  • Al-Habsy, Salim K
  • Suliman, Fakhr Eldin O
  • Al-Lawati, Haidar A J
  • 1 Department of Chemistry, College of Science, Sultan Qaboos University, Al-Khod 123, Box 36, Muscat, Sultanate of Oman. [email protected] , (Oman)
Type
Published Article
Journal
Journal of Fluorescence
Publisher
Springer-Verlag
Publication Date
Jan 01, 2012
Volume
22
Issue
1
Pages
467–474
Identifiers
DOI: 10.1007/s10895-011-0980-6
PMID: 21938387
Source
Medline
License
Unknown

Abstract

A sensitive time-resolved luminescence method for the determination of meloxicam (MX) in methanol and in aqueous solution is described. The method is based on the luminescence sensitization of europium (Eu(3+)) by formation of ternary complex with MX in the presence of 1,10-phenanthroline as coligand, Tween-80 as surfactant and gadolinium ion as a co-luminescence reagent. The signal for Eu-MX-1,10-phenanthroline is monitored at λ(ex) = 360 nm and λ(em) = 620 nm. Optimum conditions for the formation of the complex in aqueous system were 0.01 M TRIS buffer, pH 8.0, 1,10-phenanthroline (6.0 × 10(-6) M), Gd(3+) (7.0 × 10(-6) M), Tween-80 (0.28%) and 1.75 mM of Eu(3+) which allows the determination of 20-800 ppb of MX with limit of detection (LOD) of 7 ppb. The relative standard deviations of the method range between 0.1 and 1.1% indicating excellent reproducibility of the method. The proposed method was successfully applied for the assay of MX in pharmaceutical formulations, plasma and in urine samples. Average recoveries of 99.8 ± 1.1%, 100.2 ± 0.9% and 100.9 ± 1.1% were obtained for MX in tablet, plasma and urine sample respectively.

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