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Determination of LDL receptors on cultured lymphocytes using fluorescein-labeled LDL.

Authors
  • Sakai, H
  • Kaneshige, H
  • Endoh, M
Type
Published Article
Journal
American journal of clinical pathology
Publication Date
Jul 01, 1982
Volume
78
Issue
1
Pages
58–62
Identifiers
PMID: 7048895
Source
Medline
License
Unknown

Abstract

A rapid, sensitive procedure for the detection of LDL receptors on cell surface without using radioisotopes is described. Fluorescein-isothiocyanate (FITC) labeled LDL bound with cultured human lymphocytes, and the degree of that binding was compatible with that of 125 I-labeled LDL. The staining of cells with FITC-LDL allows identification and enumeration of LDL-binding cells using fluorescence microscopy or a fluorescence activated cell sorter (FACS). It is suggested that FITC-LDL can be applied for screening of patients with various degrees of activity in LDL receptors on their cell surfaces.

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