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Determination of kaempferol and quercetin in Xindakang tablet by β-cyclodextrin modified micellar electrokinetic capillary chromatography.

Authors
Type
Published Article
Journal
Pakistan journal of pharmaceutical sciences
Publication Date
Volume
27
Issue
3
Pages
487–490
Identifiers
PMID: 24811806
Source
Medline
License
Unknown

Abstract

A method was proposed to determine kaempferol and quercetin in Hippophae rhamnoides L medicinal preparation xindakang tablet by β-cyclodextrin modified micellar electrokinetic capillary chromatography. Under the optimized conditions: buffer solution of 20 mmol/L Na(2)B(4)O(7)-KH(2)PO(4) (pH 9.0)-20mmol/L SDS-6mmol/L β-CD-5%(v/v) MeCN, applied voltage of 16 kV and injection time of 8s, the two analytes were separated well within 10 minutes. The calibration was linear in the 0.02-0.80 and 0.02-0.70 mg/mL range for kaempferol and quercetin, respectively. The reproducibility based on migration time and peak height were 0.47% and1.87% for kaempferol, 0.55% and 2.02% for quercetin. The detection limits based on three times noise were 0.010 mg/mL and 0.008 mg/mL for kaempferol and quercetin, respectively. The developed method was utilized to analyze real samples and running recovery experiments with satisfactory results.

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