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Determination of hydrogen sulfide in fermentation broths containing SO 2 .

Authors
  • Acree, T E
  • Sonoff, E P
  • Splittstoesser, D F
Type
Published Article
Journal
Applied microbiology
Publication Date
Jul 01, 1971
Volume
22
Issue
1
Pages
110–112
Identifiers
PMID: 5111300
Source
Medline
License
Unknown

Abstract

A procedure for the determination of hydrogen sulfide in fermentation broths containing up to 100 mug of SO(2) per ml is described. The method involves the sparging of H(2)S from the broth into a cadmium hydroxide absorption solution, the formation of methylene blue from the absorbed sulfide, and the measuring of this color spectrophotometrically. The use of cadmium hydroxide instead of zinc acetate, the common absorbent, substantially reduced the interference of SO(2) with the analysis.

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