The determination of flux through phenylalanine hydroxylase and homogentisate oxidase in isolated hepatocytes.
- Published Article
- Publication Date
Oct 01, 1984
The metabolic fate of [4-3H]phenylalanine incubated with isolated hepatocytes has been investigated. Analysis of 3H2O production and fluorimetric estimation of tyrosine production has allowed calculation of flux through phenylalanine hydroxylase and homogentisate oxidase. The resulting flux determinations are physiologically relevant and hormonally sensitive.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/6518276